Application
Western blot analysis of LY75 Antibody (Center) (Cat. #AP8994c) in HL-60 cell line lysates (35ug/lane). LY75 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
LY75 Antibody (Center) (Cat. #AP8994c) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | LY75 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LY75; CD205; CLEC13B; Lymphocyte antigen 75; C-type lectin domain family 13 member B; DEC-205; gp200-MR6; CD_antigen=CD205; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD205 CLEC13B |
|---|---|
| Alternative Names | LY75 CD205 CLEC13B Lymphocyte antigen 75 C-type lectin domain family 13 member B DEC-205 gp200-MR6 CD_antigen=CD205 Flags: Precursor |
| Molecular Weight(MW) | 198311 Da |
| Function | Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen- processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes |
| Tissue Specificity | Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B-lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells |
| Cellular Localization | Membrane; Single-pass type I membrane protein (By similarity) |
| Entrez Gene | 4065 |
|---|
Application
Western blot analysis of LY75 Antibody (Center) (Cat. #AP8994c) in HL-60 cell line lysates (35ug/lane). LY75 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
LY75 Antibody (Center) (Cat. #AP8994c) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
