Application
Western blot analysis of IκB-ε expression in non-transfected 293T: (A) andmouse IκB-ε transfected 293T: (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining in glandular cells and lymphoid tissue at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Antibodies tested include IκB-ε (M-364): (A) and IκB-ε (M-121): (B).| Product Name | IκB-ε Antibody (M-364) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-365 representing full length IκB-ε of mouse origin |
| Modification Notes | On the basis of both functional and structural considerations, members of the I˚B family of proteins can be divided into four groups. The first of these groups, I˚B-å, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to ˚B sites, suggesting that the I˚B-å family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the I˚B family is represented by a protein designated I˚B-∫. The third group of I˚B proteins is represented by I˚B-©, which is identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and is expressed predominantly in lymphoid cells. An additional I˚B family member, I˚B-é, has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IκB-ε of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-365 representing full length I&kappa B-&epsilon of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-365 representing full length IκB-ε of mouse origin |
| Entrez Gene | 4794 |
|---|
Application
Western blot analysis of IκB-ε expression in non-transfected 293T: (A) andmouse IκB-ε transfected 293T: (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining in glandular cells and lymphoid tissue at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Antibodies tested include IκB-ε (M-364): (A) and IκB-ε (M-121): (B).| Application Notes | recommended for detection of IκB-ε of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
