Application
Western blot analysis of M-CSF (arrow) using rabbit polyclonal M-CSF Antibody (Center) (Cat. #AP6754c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MCSF gene (Lane 2) (Origene Technologies).Application
M-CSF Antibody (Center) (Cat. #AP6754c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of M-CSF Antibody (Center)(Cat#AP6754c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
M-CSF Antibody (Center) (AP6754c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of M-CSF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | M-CSF Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CSF1; Macrophage colony-stimulating factor 1; Lanimostim; Processed macrophage colony-stimulating factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIFIHC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CSF1 Macrophage colony-stimulating factor 1 Lanimostim Processed macrophage colony-stimulating factor 1 |
|---|---|
| Molecular Weight(MW) | 60179 Da |
| Function | Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration Plays a role in lipoprotein clearance |
| Tissue Specificity | This M-CSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237~266 amino acids from the Center region of human M-CSF. |
| Cellular Localization | Cell membrane; Single-pass membrane protein. |
| Entrez Gene | 1435 |
|---|
Application
Western blot analysis of M-CSF (arrow) using rabbit polyclonal M-CSF Antibody (Center) (Cat. #AP6754c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MCSF gene (Lane 2) (Origene Technologies).
Application
M-CSF Antibody (Center) (Cat. #AP6754c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of M-CSF Antibody (Center)(Cat#AP6754c) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
M-CSF Antibody (Center) (AP6754c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of M-CSF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
