Application
Western blot analysis of MTH1 expression in non-transfected 293T: (A), mouse MTH1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of MTH1 expression in non-transfected 293T: (A), human MTH1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of MTH1 expression in Jurkat whole cell lysate.| Product Name | MTH1 Antibody (H-159) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 38-197 mapping at the C-terminus of MTH1 of human origin |
| Modification Notes | MTH1, also known as NUDT1 (nudix (nucleoside diphosphate linked moiety X)-type motif 1), is a 179 amino acid cytoplasmic protein that is a member of the nudix hydrolase family. Highly expressed in testis, thymus and proliferating blood lymphocytes, MTH1 functions as an antimutagenic that hydrolyzes oxidized purine nucleoside triphosphates to their corresponding monophosphates. Through its ability to enzymatically hydrolyze ATP and GTP to AMP and GMP, respectively, MTH1 prevents misincorporation of GTP into DNA, thus preventing A:T to C:G transversions. The cytoplasmic location of MTH1, along with its antimutagenic capabilities, suggests that it may also be involved in the sanitization of nucleotide pools for both mitochondrial and nuclear genomes. Four isoforms of MTH1 exist—three of which are formed due to alternative splicing events and one of which is formed via a single-nucleotide polymorphism. Overexpression of MTH1 is implicated in prostate and cell lung carcinomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MTH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 38-197 mapping at the C-terminus of MTH1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 38-197 mapping at the C-terminus of MTH1 of human origin |
| Entrez Gene | 4521 |
|---|
Application
Western blot analysis of MTH1 expression in non-transfected 293T: (A), mouse MTH1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of MTH1 expression in non-transfected 293T: (A), human MTH1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of MTH1 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of MTH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
