Application
Western blot analysis of PAPP-A expression in non-transfected: (A) and mouse PAPP-A transfected: (B) 293 whole cell lysates.| Product Name | PAPP-A Antibody (H-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 666-840 mapping within an internal region of PAPP-A of human origin |
| Modification Notes | Pregnancy-associated plasma protein-A (Pappalysin-1 or PAPP-A), also known as Insulin-like growth factor-dependent IGF-binding protein 4 (IGFBP4) protease, is a member of the peptidase M43B family of proteins. PAPP-A, a metalloproteinase, cleaves Insulin-like growth factor binding proteins IGFBP4 and IGFBP5, releasing bound IGF. PAPP-A is primarily expressed in septa and anchoring villi in placenta and is also expressed in pregnancy serum. Levels of PAPP-A increase throughout pregnancy. Lower levels of expression can be detected in kidney, prostate, breast, ovary and endometrial tissues. PAPP-A is a secreted protein that can form homodimers; in pregnancy serum PAPP-A may also form a heterotetramer with PRG-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PAPP-A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 666-840 mapping within an internal region of PAPP-A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 666-840 mapping within an internal region of PAPP-A of human origin |
| Entrez Gene | 5069 |
|---|
Application
Western blot analysis of PAPP-A expression in non-transfected: (A) and mouse PAPP-A transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of PAPP-A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
