Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of BID expression in Jurkat whole cell lysate.Application
Western blot analysis of BID expression in HeLa (A), RAW 264.7 (B) and WEHI-231 (C) whole cell lysates.Application
Western blot analysis of BID expression in non-transfected 293T: (A), human BID transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of BID expression in non-transfected: (A) and mouse BID transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BID expression in non-transfected control (A) and BID siRNA transfected (B) HeLa cells. Blot probed with BID (FL-195): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of BID expression in non-transfected control (A) and BID siRNA transfected (B) WEHI-3 cells. Blot probed with BID (FL-195): . GAPDH (FL-335): used as specificity and loading control.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | BID Antibody (FL-195) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-195 representing full length BID of human origin |
| Modification Notes | Members of the Bcl-2 family of proteins interact to regulate programmed cell death, or apoptosis. Various homodimers and heterodimers formed by proteins in this family can either promote or inhibit apoptosis. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect on certain hematopoietic cell lines following growth factor withdrawal. Additional apoptotic inhibitors in this family include A1, Bag-1, Bcl-w, Bcl-x and Mcl-1. Pro-apoptotic members of this family include Bax, Bad, Bak, Bik (NBK) and BID. BID contains a BH3 domain which allows it to dimerize with and counter the death repressor effects of Bcl-2. BID has also been shown to heterodimerize with Bcl-x and the death agonist Bax. BID is localized predominantly in the cytosol and is also present in membrane fractions. It is highly expressed in kidney and can also be detected in brain, spleen, liver, testis and lung. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BID of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-195 representing full length BID of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-195 representing full length BID of human origin |
| Entrez Gene | 637 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of BID expression in Jurkat whole cell lysate.
Application
Western blot analysis of BID expression in HeLa (A), RAW 264.7 (B) and WEHI-231 (C) whole cell lysates.
Application
Western blot analysis of BID expression in non-transfected 293T: (A), human BID transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of BID expression in non-transfected: (A) and mouse BID transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BID expression in non-transfected control (A) and BID siRNA transfected (B) HeLa cells. Blot probed with BID (FL-195): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of BID expression in non-transfected control (A) and BID siRNA transfected (B) WEHI-3 cells. Blot probed with BID (FL-195): . GAPDH (FL-335): used as specificity and loading control.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of BID of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
