Application
Western blot analysis of cathepsin K expression in MCF7 (A), H69AR (B) and C32 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cell surface localization.| Product Name | cathepsin K Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-240 mapping within an internal region of cathepsin K of human origin |
| Modification Notes | The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O (1-6). The aspartyl protease class is composed of cathepsins D and E (7,8). Cathepsin G is in the serine protease class (9). Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsin K expression is highest in bone, cartilage and skeletal muscle. The strongest mRNA levels are revealed in osteoclasts (4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of cathepsin K of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-240 mapping within an internal region of cathepsin K of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-240 mapping within an internal region of cathepsin K of human origin |
| Entrez Gene | 1513 |
|---|
Application
Western blot analysis of cathepsin K expression in MCF7 (A), H69AR (B) and C32 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cell surface localization.| Application Notes | recommended for detection of cathepsin K of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
