Application
Western blot analysis of COPB expression in mouse kidney tissue extract.Application
Western blot analysis of COPB expression in non-transfected: (A) and mouse COPB transfected: (B) 293T whole cell lysates.| Product Name | COPB Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 654-953 mapping at the C-terminus of COPB of human origin |
| Modification Notes | Membrane and vesicular trafficking in the early secretory pathway are mediated by non-Clathrin COP (coat protein) I-coated vesicles. COPB (∫-COP) is a marker protein for pre-Golgi intermediates (vesicular tubular clusters or VTCs). GIV (Gå i/s interacting protein) co-localizes with COPB and Gå i3 on vesicles found in close proximity to ER exit sites and to cis Golgi cisternae. Afadin DIL domain-interacting protein (ADIP) co-localizes with ∫'-COP (COPP) at the Golgi complex in Madin Darby canine kidney and normal rat kidney cells. Non-Clathrin-coated vesicles mediate membrane traffic through the Golgi complex. COPB is a major component of the coat of non-Clathrin-coated vesicles. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of COPB of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 654-953 mapping at the C-terminus of COPB of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 654-953 mapping at the C-terminus of COPB of human origin |
| Entrez Gene | 1315 |
|---|
Application
Western blot analysis of COPB expression in mouse kidney tissue extract.
Application
Western blot analysis of COPB expression in non-transfected: (A) and mouse COPB transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of COPB of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
