Application
Western blot analysis of ErbB-4 phosphorylation in untreated (A), Neuregulin-1 treated (B) and Neuregulin-1 and lambda protein phosphatase (A) treated (C) T-47D whole cell lysates.Application
Western blot analysis of ErbB-4 phosphorylation in untreated (A), EGF treated (B) and EGF treated and lambda protein phosphatase treated (C) A-431 whole cell lysates.Application
Western blot analysis of ErbB-4 phosphorylation in untreated (A) and EGF-treated (B) A-431 whole cell lysates.| Product Name | p-ErbB-4 Antibody (Tyr 1056) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 1056 of ErbB-4 of human origin |
| Modification | Phosphoralated |
| Modification Notes | The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. The gene encoding ErbB-4 is expressed as a 1full length protein, which is proteolytically cleaved to produce a membrane-anchored cytoplasmic domain fragment and an ectodomain fragment. Phosphorylation of Tyr 1056 mediates the interaction of ErbB-4 with the Yes Associated Protein (YAP), which may regulate transcription. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Tyr 1056 phosphorylated ErbB-4 of r and h origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 1056 of ErbB-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 1056 of ErbB-4 of human origin |
| Entrez Gene | 2066 |
|---|
Application
Western blot analysis of ErbB-4 phosphorylation in untreated (A), Neuregulin-1 treated (B) and Neuregulin-1 and lambda protein phosphatase (A) treated (C) T-47D whole cell lysates.
Application
Western blot analysis of ErbB-4 phosphorylation in untreated (A), EGF treated (B) and EGF treated and lambda protein phosphatase treated (C) A-431 whole cell lysates.
Application
Western blot analysis of ErbB-4 phosphorylation in untreated (A) and EGF-treated (B) A-431 whole cell lysates.| Application Notes | recommended for detection of Tyr 1056 phosphorylated ErbB-4 of r and h origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
