Application
Western blot analysis of apoA-I expression in non-transfected: (A) and mouse apoA-I transfected: (B) 293T whole cell lysates.Application
Western blot analysis of apoA-I expression in human VLDL (A) and HDL (B).| Product Name | apoA-I Antibody (FL-267) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-267 representing full length apoA-I of human origin |
| Modification Notes | Apolipoproteins are protein components of plasma lipoproteins. The human apoA-I gene encodes a single chain, 243 amino acid protein which promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a cofactor for lecithin cholesterolacyltransferase (LCAT), which is responsible for the formation of most plasma cholesteryl esters. The human apoA-II gene encodes the second most abundant protein of HDL particles, where it influences plasma levels of free fatty acids (FFA). The human apoA-IV gene encodes a 396 amino acid preprotein, which after proteolytic processing is secreted from the intestine in association with chylomicron particles. ApoA-IV is a potent activator of LCAT in vitro. The human apoA-V gene encodes a 366 amino acid protein that is believed to be an important determinant of plasma triglyceride levels. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of apoA-I of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-267 representing full length apoA-I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-267 representing full length apoA-I of human origin |
| Entrez Gene | 335 |
|---|
Application
Western blot analysis of apoA-I expression in non-transfected: (A) and mouse apoA-I transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of apoA-I expression in human VLDL (A) and HDL (B).| Application Notes | recommended for detection of apoA-I of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
