Application
Western blot analysis of CRP expression in Hep G2 (A), Caki-1 (B) and Jurkat (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of CRP expression in AML-193 (A), HL-60 (B) and Daudi (C) whole cell lysates.Application
Western blot analysis of CRP expression in Hep G2 whole cell lysate.| Product Name | CRP Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 19-108 mapping near the N-terminus of CRP of human origin |
| Modification Notes | Pentraxins, which include C-reactive protein (CRP) and serum amyloid P component (SAP), are prototypic acute phase proteins. CRP and SAP are produced by liver epithelial cells and are characterized by a cyclic pentameric structure and calcium-dependent ligand binding. IL-6 is the major inducer of human CRP gene, and IL-1 and steroids can enhance this induction. Testosterone is required for the expression of CRP transgene in vivo, whereas testosterone is not required for expression of the SAP gene. During the acute-phase response, cytokine C5a acts with IL-6 and/or IL-1b to promote upregulation of the CRP and SAP genes. Both Stat3 and C/EBP are involved in mouse SAP gene expression, but only Stat3 is involved in mouse CRP gene expression. SAP binds to a variety of molecules, including autoantigens and chromatin. Both CRP and SAP also bind to Fc g R and opsonize particles for phagocytosis by human polymorphonuclear leukocytes. Opsonization of zymosan by CRP is mediated through Fc g RI, while Fc g RII and Fc g RIII are receptors for SAP. Therefore, CRP and SAP play critical roles in the host defense system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CRP of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 19-108 mapping near the N-terminus of CRP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 19-108 mapping near the N-terminus of CRP of human origin |
| Entrez Gene | 1401 |
|---|
Application
Western blot analysis of CRP expression in Hep G2 (A), Caki-1 (B) and Jurkat (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of CRP expression in AML-193 (A), HL-60 (B) and Daudi (C) whole cell lysates.
Application
Western blot analysis of CRP expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of CRP of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
