Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and membrane localization.Application
Western blot analysis of HPA1 expression in Jurkat (A) and HL-60 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and nuclear staining of glandular cells.| Product Name | HPA1 Antibody (M-45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 491-535 mapping at the C-terminus of heparanase 1 of mouse origin |
| Modification Notes | Heparanases (HPA) degrade heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix and play animportant role in the extravasation of blood-borne tumor cells and inflammatory leukocytes. HPA1 dismantles the subendothelial basal membrane and facilitates the metastasis of blood-borne tumor cells. Furthermore, HPA1 induces angiogenesis and likely promotes the vascularization of tumors. Upon degradation, HPAs free growth factors and cytokines that stimulate cell proliferation and chemotaxis. Fibroblasts endocytose extracellular HPA1 for cytoplasmic accumulation in vitro. Proteolytic processing at the cell surface of a precursor begets an active form of HPA1. The gene encoding human HPA1 maps to chromosome 4q21.3 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of heparanase 1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 491-535 mapping at the C-terminus of heparanase 1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 491-535 mapping at the C-terminus of heparanase 1 of mouse origin |
| Entrez Gene | 15442 |
|---|
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of HPA1 expression in Jurkat (A) and HL-60 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and nuclear staining of glandular cells.| Application Notes | recommended for detection of heparanase 1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
