Application
Western blot analysis of Fos B expression in non-transfected: (A) and human Fos B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Fos B expression in non-transfected: (A) and human Fos B transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded rat brain tissue showing nuclear staining of the Nucleus Accumbens area. Image kindly provided by Regina Vontell, Adrienne Betz and John Salamone, University of Connecticut.| Product Name | Fos B Antibody (H-237) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-237 of Fos B of human origin |
| Modification Notes | The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family. Murine Fos B encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B, which bind to TRE DNA response elements. Functional homologs of c-Fos and Fos B include Fra-1 and Fra-2 genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Fos B, ΔFos B and, to a lesser extent, c-Fos, Fra-1 and Fra-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-237 of Fos B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-237 of Fos B of human origin |
| Entrez Gene | 2354 |
|---|
Application
Western blot analysis of Fos B expression in non-transfected: (A) and human Fos B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Fos B expression in non-transfected: (A) and human Fos B transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded rat brain tissue showing nuclear staining of the Nucleus Accumbens area. Image kindly provided by Regina Vontell, Adrienne Betz and John Salamone, University of Connecticut.| Application Notes | recommended for detection of Fos B, ΔFos B and, to a lesser extent, c-Fos, Fra-1 and Fra-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
