Application
Western blot analysis of PDE4C expression in SH-SY5Y whole cell lysate.Application
Western blot analysis of PDE4C expression in non-transfected: (A) and mouse PDE4C transfected: (B) 293T whole cell lysates.| Product Name | PDE4C Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 673-712 mapping at the C-terminus of PDE4C of human origin |
| Modification Notes | Phosphodiesterases (PDE) hydrolyze cAMP to 5’AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families (1). A unique upstream conserved region (UCR) characterizes the PDE4 family (2). Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both UCR domains are necessary for dimerization of PDE4 isoforms (3). The human PDE4C gene maps to chromosome 19p13.1 (4). The splice variants include isoforms PDE4C1-7 (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PDE4C of mouse and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 673-712 mapping at the C-terminus of PDE4C of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 673-712 mapping at the C-terminus of PDE4C of human origin |
| Entrez Gene | 5143 |
|---|
Application
Western blot analysis of PDE4C expression in SH-SY5Y whole cell lysate.
Application
Western blot analysis of PDE4C expression in non-transfected: (A) and mouse PDE4C transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PDE4C of mouse and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
