Application
Western blot analysis of DNA Ligase IV expression in HeLa (A) and Ramos (B) whole cell lysates.Application
Western blot analysis of DNA Ligase IV expression in HeLa whole cell lysate.Application
Western blot analysis of human recombinant DNA Ligase IV fusion protein.| Product Name | DNA Ligase IV Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 545-844 mapping at the C-terminus of DNA Ligase IV of human origin |
| Modification Notes | The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These proteins form a critical complex consisting of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligate accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4/ DNA Ligase IV complex is also involved in V(D)J recombination. V(D)J recombination occurs in normal development of the adaptive immune system and involves the formation of a double-strand break intermediate. Deletions of either DNA Ligase IV or XRCC4 inhibit the completion of V(D)J recombination, resulting in a high incidence of apoptosis in the developing nervous system and a block in B and T cell maturation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DNA Ligase IV of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 545-844 mapping at the C-terminus of DNA Ligase IV of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 545-844 mapping at the C-terminus of DNA Ligase IV of human origin |
| Entrez Gene | 3981 |
|---|
Application
Western blot analysis of DNA Ligase IV expression in HeLa (A) and Ramos (B) whole cell lysates.
Application
Western blot analysis of DNA Ligase IV expression in HeLa whole cell lysate.
Application
Western blot analysis of human recombinant DNA Ligase IV fusion protein.| Application Notes | recommended for detection of DNA Ligase IV of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
