Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of macrophages and pneumocytes.Application
Western blot analysis of AMPKα1/2 expression in K-562 (A) and C2C12 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed L8 cells showing cytoplasmic localization.Application
Western blot analysis of AMPKα1 and AMPKα2 expression in L8 whole cell lysate.| Product Name | AMPKα1/2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 251-550 mapping at the C-terminus of AMPKα1 of human origin |
| Modification Notes | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic å subunit and regulatory ∫ and © subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKå1 and AMPKå2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPK∫1 encodes a 271 amino acid protein and human AMPK∫2 encodes a 272 amino acid protein. The human AMPK©1 gene encodes a 331 amino acid protein. Human AMPK©2 and AMPK©3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of AMPKα1 and AMPKα2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 251-550 mapping at the C-terminus of AMPK&alpha 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 251-550 mapping at the C-terminus of AMPKα1 of human origin |
| Entrez Gene | 5562 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of macrophages and pneumocytes.
Application
Western blot analysis of AMPKα1/2 expression in K-562 (A) and C2C12 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed L8 cells showing cytoplasmic localization.
Application
Western blot analysis of AMPKα1 and AMPKα2 expression in L8 whole cell lysate.| Application Notes | recommended for detection of AMPKα1 and AMPKα2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
