Application
Western blot analysis of LARG expression in non-transfected: (A) and human LARG transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of LARG expression in HeLa whole cell lysate.| Product Name | LARG Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-70 of LARG of human origin |
| Modification Notes | Leukemia-associated RhoGEF (LARG) is a 1,544 amino acid, guanine nucleotide exchange factor (GEF) that contains a PDZ domain, a LH/RGS domain, and a Dbl homology/ pleckstrin homology domain (1-3). LARG shares homology with other RhoGEFs, including such oncogenes as DBL, VAV1, TIAM, and BCR (4). RhoGEFs containing RGS domains are capable of associating with activated Galpha subunits, and can function as GTPase activating proteins (GAPs) (5). LARG transcripts have been detected in human peripheral blood leukocytes, spleen, prostate, testis, ovary, small intestine, colon, and thymus (1). The LARG protein may elicit signals through a G protein-coupled receptor (GPCR)-Rho-dependent signaling pathway (1). Genetic alterations that occur at human chromosome position 11q23, where LARG maps, are prevalent in acute leukemias (1,4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LARG of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-70 of LARG of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-70 of LARG of human origin |
| Entrez Gene | 23365 |
|---|
Application
Western blot analysis of LARG expression in non-transfected: (A) and human LARG transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of LARG expression in HeLa whole cell lysate.| Application Notes | recommended for detection of LARG of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
