Application
Western blot analysis of an Omni fusion protein using Omni-probe (M-21): antibody at IgG concentrations of 1.0 to 0.02 µg/ml.| Product Name | Omni-probe Antibody (M-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping between the (His)6 and polylinker sequence of Xpress series of prokaryotic and eukaryotic exspression vectors |
| Modification Notes | Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coli, insect and mammalian hosts are in common usage. Such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins (1). For instance, the prokaryotic pRSET A, B, C and pTrc His A, B, C; baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors encode fusion proteins containing polyhistidine sequences fused to insertion cDNA sequences allowing for rapid purification on nickel-charged agarose resin (2-4). Omni-probe antibodies directed to specific vector sequences mapping between the polyhistidine tag and cDNA insert sequences provide a convenient and rapid means for identification of expressed fusion proteins in different hosts. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of fusion proteins encoded by the prokaryotic pRSET A, B, C and pTrc His A, B, C baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping between the (His)6 and polylinker sequence of Xpress series of prokaryotic and eukaryotic exspression vectors |
|---|---|
| Tissue Specificity | epitope mapping between the (His)6 and polylinker sequence of Xpress series of prokaryotic and eukaryotic exspression vectors |
Application
Western blot analysis of an Omni fusion protein using Omni-probe (M-21): antibody at IgG concentrations of 1.0 to 0.02 µg/ml.| Application Notes | recommended for detection of fusion proteins encoded by the prokaryotic pRSET A, B, C and pTrc His A, B, C baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
