| Product Name | Mpk1 Antibody (y-244) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 241-484 of Mpk1 of Saccharomyces cerevisiae origin |
| Modification Notes | Yeasts maintain the integrity of their cell walls via a MAP kinase cascade. This cascade consists of a MAP kinase (mitogen-activated protein kinase, also called ERK, for extracellular regulated kinase) as well as several upstream regulatory kinases (MAPKKs or MEKs, for MAP/ERK kinase) (1,2). Pkc1 (also designated Sst1), a yeast homolog of the mammalian PKC a, b and g isoforms, transmits extracellular signals to Bck1, a MAPKKK (also called Slk1, Ssp31 or Las3) (3,4). Bck1 then activates two MAPKKs, Mkk1 and Mkk2 (also referred to as Ssp32 and Ssp33, respectively) (5-7). These in turn activate the MAP kinase Mpk1 (also called Slt2) (8). Mutants lacking any component of this cascade exhibit a defect in cell lysis resulting from deficient cell wall synthesis. Bck2 (also designated Ctr7) has been identified as a suppressor of Pkc1 and Mpk1 deletions (9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of Mpk1 of Saccharomyces cerevisiae origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 241-484 of Mpk1 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 241-484 of Mpk1 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of Mpk1 of Saccharomyces cerevisiae origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
