Application
Western blot analysis of 20 ng purified human recombinant OSM using primary antibody at 0.1 µg/ml.| Product Name | OSM Antibody (N-1) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the N-terminus of OSM of human origin |
| Modification Notes | Oncostatin M (OSM) is a glycoprotein that was originally isolated from the conditioned medium of U937 human histiocytic leukemia cells that had been induced to differentiate into macrophage-like cells by treatment with phorbol 12-myristate 13 acetate. OSM inhibits the growth of a broad range of human tumor cell lines, but does not influence the growth of normal human fibroblasts. High-affinity binding sites for OSM have been detected on normal and tumor cells, and a receptor has been identified by chemical cross-linking studies. Expression of OSM is greatest in activated monocytic and lymphocytic cell lines and in normal adherent macrophages. Amino acid sequence analysis of OSM has revealed homology with leukemia inhibitory factor (LIF), granulocyte colony stimulating factor (G-CSF) and interleukin 6 (IL-6), all of which affect the growth and differentiation of a broad range of cell types, including those of hematopoietic origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of OSM of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the N-terminus of OSM of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the N-terminus of OSM of human origin |
| Entrez Gene | 5008 |
|---|
Application
Western blot analysis of 20 ng purified human recombinant OSM using primary antibody at 0.1 µg/ml.| Application Notes | recommended for detection of OSM of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
