Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human uterus tissue showing cytoplasmic and nuclear staining of glandular cells.Application
Western blot analysis of connexin 36 expression in rat adrenal gland tissue extract (A) and PC-12 whole cell lysate (B).| Product Name | connexin 36 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-210 mapping to an internal region of connexin 36 of human origin |
| Modification Notes | The connexin family of proteins form hexameric complexes called “connexons” that facilitate movement of low molecular weight proteins between cells via gap junctions (1). Connexin proteins share a common topology of 4 transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop, and cytoplasmic N- and C-termini (2). Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions (2). Each of the approximately 20 connexin isoforms produces channels with distinct permeabilities and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another (2). Consequently, a wide variety of malignant phenotypes associate with decreased connexin expression and gap junction communication, dependent on the particular connexin that is effected (3, 2). For instance, global ischemia induces connexin 36 expression, implying a role for this connexin in interneuronal survival (4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of connexin 36 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-210 mapping to an internal region of connexin 36 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-210 mapping to an internal region of connexin 36 of human origin |
| Entrez Gene | 57369 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human uterus tissue showing cytoplasmic and nuclear staining of glandular cells.
Application
Western blot analysis of connexin 36 expression in rat adrenal gland tissue extract (A) and PC-12 whole cell lysate (B).| Application Notes | recommended for detection of connexin 36 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
