Application
Western blot analysis of FLASH expression in mouse heart tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in molecular and granular layers and Purkinje cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization (A) and nuclear localization after UV exposure (B).| Product Name | FLASH Antibody (M-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1663-1962 mapping at the C- terminus of FLASH of mouse origin |
| Modification Notes | Fas is a member of the tumor necrosis factor family of membrane receptors, which induces apoptosis by binding to its ligand, Fas-L. Fas mediates apoptosis through a group of proteins that bind to its intracellular “death” domain, including FADD. After binding to Fas, FADD binds to caspase-8, resulting in activation of caspase-8 and the initiation of the caspase-mediated apoptotic pathway. FLASH, for FLICE- associated huge protein, has been identified as an additional component of the Fas-FADD-caspase-8 complex, also referred to as the DISC complex. FLASH shares homology with the C. elegans CED-4 protein and the mammalian Apaf-1 protein, which are both involved in activating caspases. FLASH was shown to be required for activation of caspase-8 during Fas-mediated apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FLASH of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1663-1962 mapping at the C- terminus of FLASH of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1663-1962 mapping at the C- terminus of FLASH of mouse origin |
| Entrez Gene | 9994 |
|---|
Application
Western blot analysis of FLASH expression in mouse heart tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in molecular and granular layers and Purkinje cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization (A) and nuclear localization after UV exposure (B).| Application Notes | recommended for detection of FLASH of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
