Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells and interstitial cells.Application
Western blot analysis of PRC1 expression in non-transfected 293T: (A), mouse PRC1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.Application
Western blot analysis of PRC1 expression in HeLa whole cell lysate (A) and mouse embryo tissue extract (B).| Product Name | PRC1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 451-620 of PRC1 of human origin |
| Modification Notes | Sequential activation and inactivation of Cdk/cyclin complexes regulates the cell cycle. PRC1 (for protein regulating cytokinesis 1) has been identified as a substrate for several Cdks, including Cdc2 and Cdk2. PRC1 binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis. The yeast homolog of PRC1, Ase1, is essential for spindle assembly, elongation and disassembly during mitosis. Ase1 has been shown to undergo degradation mediated by the APC (anaphase-promoting complex) upon entry into G1 phase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PRC1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 451-620 of PRC1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 451-620 of PRC1 of human origin |
| Entrez Gene | 9055 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells and interstitial cells.
Application
Western blot analysis of PRC1 expression in non-transfected 293T: (A), mouse PRC1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
Application
Western blot analysis of PRC1 expression in HeLa whole cell lysate (A) and mouse embryo tissue extract (B).| Application Notes | recommended for detection of PRC1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
