Application
Western blot analysis of Hemoglobin β/γ/δ expression in mouse heart tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) programApplication
Western blot analysis of Hemoglobin β/γ/δ expression in human peripheral blood lymphocyte lysate.| Product Name | Hemoglobin β/γ/δ Antibody (H-76) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 67-147 mapping near the C-terminus of Hemoglobin β of human origin |
| Modification Notes | Hemoglobin (Hgb) is coupled to four iron-binding, methene-linked tetrapyrrole rings (heme). The å (16p13.3; 5'-Ω-pseudoz-pseudo å2-pseudo å1-å2-å1-œ1-3') and ∫ (11p15.5) globin loci determine the basic hemoglobin structure. The globin portion of hemoglobin consists of two å chains and two ∫ chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between å and ∫ chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the å1-∫2 cleavage plane. When the two å1-∫2 interfaces are closely bound, hemoglobin has a low affinity for oxygen. Hb A, which contains two å chains plus two ∫ chains, comprises 97% of total circulating hemoglobin. The remaining 3% of total circulating hemoglobin is comprised of Hb A-2, which consists of two å chains plus two ∂ chains, and fetal hemoglobin (Hb F), which consists of two å chains together with two © chains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Hemoglobin β, δ and γ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with Hemoglobin ε also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 67-147 mapping near the C-terminus of Hemoglobin &beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 67-147 mapping near the C-terminus of Hemoglobin β of human origin |
| Entrez Gene | 3043 |
|---|
Application
Western blot analysis of Hemoglobin β/γ/δ expression in mouse heart tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program
Application
Western blot analysis of Hemoglobin β/γ/δ expression in human peripheral blood lymphocyte lysate.| Application Notes | recommended for detection of Hemoglobin β, δ and γ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA may cross-react with Hemoglobin ε also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
