Application
Western blot analysis of UNC45A expression in non-transfected: (A) and mouse UNC45A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of UNC45A expression in non-transfected: (A) and human UNC45A transfected: (B) 293T whole cell lysates.| Product Name | UNC45A Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-295 mapping within an internal region of UNC45A of human origin |
| Modification Notes | UNC45A (unc-45 homolog A), also known as SMAP1 (Smooth muscle cell-associated protein 1) or GCUNC45, is a 944 amino acid protein that localizes to the perinuclear region of the cytoplasm and contains three TPR repeats. Expressed in spinal cord, bone marrow, peripheral blood leukocytes, trachea, stomach and adrenal gland, UNCA45A functions as a chaperone for HSP 90, effectively facilitating the association of HSP 90 with PR (progesterone receptor). Additionally, UNC45A is thought to be necessary for proper folding of Myosin and is required for normal myotube formation during muscle cell development, thereby playing an important role in muscle formation. Overexpression of UNC45A is associated with rapid cell proliferation and motility, especially that observed in ovarian cancer, suggesting a role for UNC45A in tumorigenesis. Multiple isoforms of UNC45A exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of UNC45A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-295 mapping within an internal region of UNC45A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-295 mapping within an internal region of UNC45A of human origin |
| Entrez Gene | 55898 |
|---|
Application
Western blot analysis of UNC45A expression in non-transfected: (A) and mouse UNC45A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of UNC45A expression in non-transfected: (A) and human UNC45A transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of UNC45A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
