Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear envelope and cytoplasmic staining of gladular cells.Application
Western blot analysis of emerin expression in non-transfected 293T: (A), human emerin transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and emerin siRNA silenced HeLa (B) cells showing diminished nuclear envelope staining in the siRNA silenced cells. Cells probed with emerin (FL-254): .Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization.Application
Western blot analysis of emerin expression in HeLa (A), Saos-2 (B) and K-562 (C) whole cell lysates.| Product Name | emerin Antibody (FL-254) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 3-254 of emerin of human origin |
| Modification Notes | Emerin is believed to be a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreyfuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Research has demonstrated that the lack of emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherentes in the heart. This may account for conduction defects in patients with EDMD. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of emerin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 3-254 of emerin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 3-254 of emerin of human origin |
| Entrez Gene | 2010 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear envelope and cytoplasmic staining of gladular cells.
Application
Western blot analysis of emerin expression in non-transfected 293T: (A), human emerin transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and emerin siRNA silenced HeLa (B) cells showing diminished nuclear envelope staining in the siRNA silenced cells. Cells probed with emerin (FL-254): .
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization.
Application
Western blot analysis of emerin expression in HeLa (A), Saos-2 (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of emerin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
