Application
Western blot analysis of CD19 expression in K-562 (A), BJAB (B), NAMALWA (C), GA-10 (D) and Daudi (E) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed BJAB cells showing membrane localization.| Product Name | CD19 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of CD19 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains (1,2). CD19 is selectively expressed on the cell surface of B-lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways (3). Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK) (4). Expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells (5). CD19 forms functional complexes with B-lymphocyte surface proteins, including integrin b1, CD21 and CD81, which are involved in regulating B-cell development (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of CD19 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of CD19 of human origin |
| Entrez Gene | 930 |
|---|
Application
Western blot analysis of CD19 expression in K-562 (A), BJAB (B), NAMALWA (C), GA-10 (D) and Daudi (E) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed BJAB cells showing membrane localization.| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
