Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining.Application
Western blot analysis of nm23-H1 expression in HeLa (A), A-431 (B), Jurkat (C), K-562 (D) and BJAB (E) whole cell lysates.| Product Name | nm23-H1 Antibody (C-20) TRITC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of nm23-H1 of human origin |
| Modification Notes | The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease-hypersensitive elements at positions 142 to 115 of the human C-Myc promotor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of nm23-H1 and, to a lesser extent, nm23-H2 of h and r origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of nm23-H1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of nm23-H1 of human origin |
| Entrez Gene | 4830 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining.
Application
Western blot analysis of nm23-H1 expression in HeLa (A), A-431 (B), Jurkat (C), K-562 (D) and BJAB (E) whole cell lysates.| Application Notes | recommended for detection of nm23-H1 and, to a lesser extent, nm23-H2 of h and r origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
