Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of GABAA Rπ expression in non-transfected: (A) and mouse GABAA Rπ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GABAA Rπ expression in non-transfected 293T: (A), mouse GABAA Rπ transfected 293T: (B) and LNCaP (C) whole cell lysates.Application
Western blot analysis of GABAA Rπ expression in MES-SA/Dx5 (A) and JAR (B) whole cell lysates.Application
Western blot analysis of GABAA Rπ expression in MES-SA/Dx5 (A) and LNCaP (B) whole cell lysates.| Product Name | GABAA Rπ Antibody (H-115) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 326-440 mapping at the C-terminus of GABAA Rπ of human origin |
| Modification Notes | GAD-65 and GAD-67, glutamate decarboxylases function to catalyze the production of GABA (γ-aminobutyric acid). In the central nervous system GABA functions as the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. Both GABAA and GABAC are ligand-gated ion channels, however, they are structurally and functionally distinct. Members of the GABAA receptor family include GABAA Rå1-6, GABAA R ∫1-3, GABAA R©1-3, GABAA R∂, GABAA Ré, GABAA Rπ, GABAA Rρ1 and GABAA Rρ2. In the uterus, the function of the π receptor appears to be related to tissue contractility. The binding of this π subunit with other GABAA receptor subunits alters the sensitivity of recombinant receptors to modulatory agents such as pregnanolone. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GABAA Rπ of mouse, rat and human origin by WB, IP, IF,IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 326-440 mapping at the C-terminus of GABAA R&pi of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 326-440 mapping at the C-terminus of GABAA Rπ of human origin |
| Entrez Gene | 2568 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of GABAA Rπ expression in non-transfected: (A) and mouse GABAA Rπ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GABAA Rπ expression in non-transfected 293T: (A), mouse GABAA Rπ transfected 293T: (B) and LNCaP (C) whole cell lysates.
Application
Western blot analysis of GABAA Rπ expression in MES-SA/Dx5 (A) and JAR (B) whole cell lysates.
Application
Western blot analysis of GABAA Rπ expression in MES-SA/Dx5 (A) and LNCaP (B) whole cell lysates.| Application Notes | recommended for detection of GABAA Rπ of mouse, rat and human origin by WB, IP, IF,IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
