Application
Antibodies tested include p-ARK-1 (95.Thr 288): (A,B,C) and ARK-1 (H-130): (D,E,F).Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human ARK-1 transfected HEK 293 cells (B).Application
Western blot analysis of ARK-1 expression in non-transfected: (A) and human ARK-1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of ARK-1 expression in non-transfected: (A) and mouse ARK-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ARK-1 expression in non-transfected: (A) and human ARK-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ARK-1 expression in HeLa whole cell lysate.Application
Western blot analysis of ARK-1 expression in BT-20 (A) and HeLa (B) whole cell lysates.| Product Name | ARK-1 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-130 of ARK-1 of human origin |
| Modification Notes | Aurora related kinase-1 (ARK-1, STK15, Aurora2, Aik1) and -2 (ARK-2, STK12, Aurora1) are centrosome-associated serine/threonine kinases that regulate centrosome separation, bipolar spindle assembly and chromosome segregation during mitosis. ARK-1 and -2 are expressed in the nucleus and localize to distinct portions of mitotic machinery such as the centrosome, spindle poles (ARK-1) and midbody (ARK-2) during mitosis. ARK-1 and -2 transcripts are present at high levels in human thymus and fetal liver. ARK-1 protein has elevated expression in colon carcinoma lines (HT-29, SNU-C2B, COLO 205, SW480, 837 and 948) and accumulates during metaphase in HeLa cells. ARK-2 protein levels are maximal during both S and G2/M phases, whereas ARK-1 protein is degraded after G2/M via the ubiquitin-proteasome pathway. ARK-2 has a unique genetic loci relative to ARK-1, suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ARK-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-130 of ARK-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-130 of ARK-1 of human origin |
| Entrez Gene | 6790 |
|---|
Application
Antibodies tested include p-ARK-1 (95.Thr 288): (A,B,C) and ARK-1 (H-130): (D,E,F).
Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human ARK-1 transfected HEK 293 cells (B).
Application
Western blot analysis of ARK-1 expression in non-transfected: (A) and human ARK-1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of ARK-1 expression in non-transfected: (A) and mouse ARK-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ARK-1 expression in non-transfected: (A) and human ARK-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ARK-1 expression in HeLa whole cell lysate.
Application
Western blot analysis of ARK-1 expression in BT-20 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of ARK-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
