Application
Western blot analysis of Perforin 1 expression in Jurkat (A), NK-92 (B) and SUP-T1 (C) whole cell lysates and rat brain (D) and mouse brain (E) tissue extracts.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic localization.| Product Name | Perforin 1 Antibody (H-315) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 241-555 mapping at the C-terminus (h) |
| Modification Notes | The major defense of the body against virus-infected and tumorigenic cells is cytotoxic T lymphocyte (CTL)-mediated cytotoxicity, which also plays a role in autoimmune diseases and transplant rejection. During CTL-mediated cytotoxicity, CTL granules containing perforin are exocytosed. Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells by forming transmembrane channels in target cell membranes. Perforin is primarily expressed in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, but has also been observed in an astrocyte population of the human brain. It has been shown that abrogation of perforin function by Ca2+-complexing agents leads to decreased levels of necrosis, demonstrating that both necrosis and apoptosis contribute to CTL-mediated cytotoxicity. Perforin activity has been shown to be induced by IL-2, IL-3, IL-4, IL-6 and to a lesser degree, TNF and IFN-g. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Perforin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 241-555 mapping at the C-terminus (h) |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 241-555 mapping at the C-terminus (h) |
| Entrez Gene | 5551 |
|---|
Application
Western blot analysis of Perforin 1 expression in Jurkat (A), NK-92 (B) and SUP-T1 (C) whole cell lysates and rat brain (D) and mouse brain (E) tissue extracts.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic localization.| Application Notes | recommended for detection of Perforin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
