Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic and nuclear staining of squamous epithelial cells.Application
Western blot analysis of Arc expression in non-transfected: (A) and human Arc transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Arc expression in non-transfected: (A) and human Arc transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnifica?tion). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse heart frozen section showing cytoplasmic staining.Application
Western blot analysis of Arc expression in PC-12 whole cell lysate (A) and mouse brain extract (B).| Product Name | Arc Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of Arc (activity-regulated cytoskeleton-associated protein) of human origin |
| Modification Notes | Growth factor stimulation has been shown to induce the expression of immediate early genes in non-neuronal cells, which encode a variety of molecules that are potentially involved in long-term cellular responses. Similar responses induced by neurotransmitter stimulation have also been seen in neuronal cells and evidence suggests that protein synthesis is required for long-term synaptic plasticity. Arc (for activity-regulated cytoskeleton-associated protein) is a growth factor and immediate early gene that is enriched in brain. Arc mRNA and protein levels are induced by neuronal activity, which is necessary to stimulate neuroplasticity, indicating a potential role for Arc in activity-dependent changes in dendrite function. Arc expression has been detected in neuronal cell bodies and dendrites in the hippocampus, amygdala, hypothalamus, striatum and cortex. Arc has been shown to localize to the cytoskeleton of neuronal cells and appears to colocalize with F-Actin, although it may associate with an Actin-associated protein rather than directly with F-Actin. It has been shown that cocaine-stimulated neuronal activity results in increased Arc mRNA levels in striatum. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Arc of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of Arc (activity-regulated cytoskeleton-associated protein) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of Arc (activity-regulated cytoskeleton-associated protein) of human origin |
| Entrez Gene | 23237 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic and nuclear staining of squamous epithelial cells.
Application
Western blot analysis of Arc expression in non-transfected: (A) and human Arc transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Arc expression in non-transfected: (A) and human Arc transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnifica?tion). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse heart frozen section showing cytoplasmic staining.
Application
Western blot analysis of Arc expression in PC-12 whole cell lysate (A) and mouse brain extract (B).| Application Notes | recommended for detection of Arc of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
