Application
Western blot analysis of NCS-1 expression in PC-12 (A), U-87 MG (B), T98G (C) and SK-N-SH (D) whole cell lysates and rat cerebellum (E) and mouse brain (F) tissue extracts.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | NCS-1 Antibody (FL-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-190 representing full length NCS-1 |
| Modification Notes | NCS-1 (for neuronal calcium sensor-1, also designated frequenin) belongs to a highly conserved family of EF-hand-containing Ca++-binding proteins expressed mainly in neurons. NCS-1 is localized to neuronal cell bodies and axons throughout the brain and spinal cord. It is also expressed in glial cells and in neuroendocrine bovine adrenal chromaffin and PC12 cells. NCS-1 is a regulatory protein involved in Ca++-dependent exocytosis of synaptic vesicles and dense core granules. NCS-1 also functions in the voltage-independent autocrine pathway that negatively regulates non-L-type Ca++ channels. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NCS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-190 representing full length NCS-1 |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-190 representing full length NCS-1 |
| Entrez Gene | 23413 |
|---|
Application
Western blot analysis of NCS-1 expression in PC-12 (A), U-87 MG (B), T98G (C) and SK-N-SH (D) whole cell lysates and rat cerebellum (E) and mouse brain (F) tissue extracts.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of NCS-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
