Application
Western blot analysis of HTR3D expression in SW480 (A), COLO 320DM (B) and HCT 116 (C) whole cell lysates.| Product Name | HTR3D Antibody (K-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within a cytoplasmic domain of HTR3D of human origin |
| Modification Notes | Serotonin is a monoamine neurotransmitter that is made in serotonergic neurons in the CNS (central nervous system) and is important in the regulation of mood, sleep, vomiting, sexuality and appetite. 5-HT3 (5-hydroxytryptamine-3) receptor is the only ligand-gated ion channel within the family of serotonin receptors. It is composed of five subunits consisting of SR-3A, SR-3B, HTR3C, HTR3D and HTR3E. HTR3D (5-hydroxytryptamine receptor 3D), also known as Serotonin receptor 3D, is a 454 amino acid multi-pass membrane protein that is one components of the pentaheteromeric complex that forms the 5-HT3 receptor. HTR3D must be co-expressed with SR-3A to form a functional 5-HT3 receptor complex on the plasma membrane. Until it is complexed with SR-3A, HTR3D is localized within the endoplasmic reticulum. Expression of HTR3D is restricted to kidney, colon and liver. There are three different isoforms of HTR3D that are expressed as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HTR3D and HTR3E of human origin by WB, IP, IF and ELISA non cross-reactive with HTR3C: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a cytoplasmic domain of HTR3D of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a cytoplasmic domain of HTR3D of human origin |
| Entrez Gene | 200909 |
|---|
Application
Western blot analysis of HTR3D expression in SW480 (A), COLO 320DM (B) and HCT 116 (C) whole cell lysates.| Application Notes | recommended for detection of HTR3D and HTR3E of human origin by WB, IP, IF and ELISA non cross-reactive with HTR3C: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
