Application
Western blot analysis of phosphorylated 14-3-3 ζ expression in untreated (A) and TNF-alpha;- rtreated(B) NIH/3T3 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-14-3-3 ζ Antibody (Ser 58) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 ∫, ©, é, Ω, ñ, † and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 58 phosphorylated 14-3-3 &zeta of mouse, rat and human origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 58 of 14-3-3 &zeta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 58 of 14-3-3 ζ of human origin |
| Entrez Gene | 7534 |
|---|
Application
Western blot analysis of phosphorylated 14-3-3 ζ expression in untreated (A) and TNF-alpha;- rtreated(B) NIH/3T3 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Ser 58 phosphorylated 14-3-3 &zeta of mouse, rat and human origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
