Application
Western blot analysis of c-Src phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase treated (C) Jurkat whole cell lysates.Application
Western blot analysis of c-Src phosphorylation in untreated (A), EGF treated (B) and EGF and lambda protein phosphatase treated (C) HEK293 whole cell lysates.| Product Name | p-c-Src Antibody (Tyr 419) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The major translational products of the Src gene family are membrane-associated tyrosine protein kinases that lack transmembrane and external amino acid sequences. By virtue of their common structural motifs, the Src family is composed of nine members in vertebrates, including c-Src, c-Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck and Blk. Src family kinases, which contain an amino-terminal cell membrane anchor followed by SH3 and SH2 domains, transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src family members are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular locations of Src family kinases may be important for the regulation of specific cellular processes, such as mitogenesis, cytoskeletal organization and membrane trafficking. c-Src (also designated pp60Src, Src p60 and proto-oncogene tyrosine protein kinase Src) is expressed in a broad range of tissue and cell types, although the highest levels of c-Src are detected in neuronal tissues and platelets. c-Src may play a role in events associated with both neuronal differentiation and maintenance of mature neuronal cell functions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Tyr 419 phosphorylated c-Src of human and rat origin, Tyr 424 phosphorylated c-Src of mouse origin, and Tyr 416 phosphorylated c-Src of avian origin by WB, IP, IF and IHC(P) also recommended for detection of correspondingly phosphorylated Fyn, c-Yes, Lyn, Lck, and Hck of human, mouse, and rat origin | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 419 of c-Src of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 419 of c-Src of human origin |
| Entrez Gene | 6714 |
|---|
Application
Western blot analysis of c-Src phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
Application
Western blot analysis of c-Src phosphorylation in untreated (A), EGF treated (B) and EGF and lambda protein phosphatase treated (C) HEK293 whole cell lysates.| Application Notes | recommended for detection of Tyr 419 phosphorylated c-Src of human and rat origin, Tyr 424 phosphorylated c-Src of mouse origin, and Tyr 416 phosphorylated c-Src of avian origin by WB, IP, IF and IHC(P) also recommended for detection of correspondingly phosphorylated Fyn, c-Yes, Lyn, Lck, and Hck of human, mouse, and rat origin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
