Application
Antibodies tested include p-PKR (Thr 451): (A,B,C) and PKR (B-10): (D,E,F).Application
Antibodies tested include p-PKR (Thr 451): (A,B,C) and PKR (M-515): (D,E,F).Application
Western blot analysis of PKR phosphorylation in untreated (A), calyculin A and IFNα treated (B) and calyculin A, IFNα and lambda protein phosphatase treated (C) HeLa whole cell lysates.Application
Western blot analysis of PKR phosphorylation in non-transfected: (A) and mouse PKR transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PKR phosphorylation in untreated (A), calyculin A treated (B) and calyclulin A and lambda protein phosphatase treated (C) HeLa whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic staining.| Product Name | p-PKR Antibody (Thr 451) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | An interferon-inducible, RNA-dependent protein serine/threonine kinase, PKR has various designations. Mouse PKR is known as DAI, dsJ, PI kinase, p65, p67 or TIK, whereas human PKR is known as p68 or p69. PKR phosphorylates its substrate, a subunit of protein synthesis initiation factor eIF-2 on Ser 51 to inhibit translation. PKR contains two dsRNA binding motifs required for its activation by dsRNA. Three kinds of regulation of PKR enzymatic activity occur, and these include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation, and posttranslational regulation by RNA mediated autophosphorylation. Human PKR contains at least 15 autophosphorylation sites, but only Thr-446 and Thr-451 in the activation loop are critical for its kinase activity. Thr-446 is the in vivo autophosphorylation site of PKR. Mutation of threonine to alanine at position 446 substantially reduces PKR function, and mutant kinase containing Ala-451 is completely inactive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 451 phosphorylated PKR of human origin and correspondingly phosphorylated Thr 414 of mouse origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 451 of PKR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 451 of PKR of human origin |
| Entrez Gene | 5610 |
|---|
Application
Antibodies tested include p-PKR (Thr 451): (A,B,C) and PKR (B-10): (D,E,F).
Application
Antibodies tested include p-PKR (Thr 451): (A,B,C) and PKR (M-515): (D,E,F).
Application
Western blot analysis of PKR phosphorylation in untreated (A), calyculin A and IFNα treated (B) and calyculin A, IFNα and lambda protein phosphatase treated (C) HeLa whole cell lysates.
Application
Western blot analysis of PKR phosphorylation in non-transfected: (A) and mouse PKR transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PKR phosphorylation in untreated (A), calyculin A treated (B) and calyclulin A and lambda protein phosphatase treated (C) HeLa whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Thr 451 phosphorylated PKR of human origin and correspondingly phosphorylated Thr 414 of mouse origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
