| Product Name | E-Ras Antibody (H-48) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 186-233 mapping at the C-terminus of E-Ras of human origin |
| Modification Notes | The Ras-encoded family of proteins bind to GDP and to GTP with high affinity. They possess a low level of intrinsic GTPase activity that can be stimulated more than 100-fold by interaction with cytosolic GTPase activating protein (GAP). Ras family members include H-Ras, K-Ras, N-Ras, M-Ras, R-Ras, E-Ras, Rheb, TC 21, RASL11B and Rad GTPase. H-Ras and K-Ras were first identified as oncogenes of acutely transforming RNA tumor viruses. Subsequently, mutated Ras genes have been found in many human tumors, providing evidence for a common genetic target in cancer. In mammals, a variety of extracellular growth factors that act through protein tyrosine kinase receptors, such as insulin, platelet-derived growth factor and nerve growth factor, require Ras to exert their effects. Embryonic stem cell-expressed Ras (E-Ras) is a 277 amino acid protein that localizes to the cytoplasmic membrane and shares 43%, 46% and 47% identity with H-Ras, K-Ras and N-Ras, respectively. E-Ras contains five highly conserved domains essential for small G proteins and a CAAX motif. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of E-Ras of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 186-233 mapping at the C-terminus of E-Ras of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 186-233 mapping at the C-terminus of E-Ras of human origin |
| Entrez Gene | 3266 |
|---|
| Application Notes | recommended for detection of E-Ras of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
