Application
Western blot analysis of TPSB2 expression in non-transfected 293T: (A), human TPSB2 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Mast Cell Tryptase expression in non-transfected: (A) and human Mast Cell Tryptase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of purified tryptase from human lung.| Product Name | Mast Cell Tryptase Antibody (FL-275) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-275 representing full length Mast Cell Tryptase of human origin |
| Modification Notes | Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Mast cell chymase, known as CMA1, is a major secreted serine protease that is involved in vasoactive peptide generation, extracellular matrix degradation and regulation of gland secretion. The human chymase gene, which maps to human chromosome 14q11.2, encodes a preproenzyme with a 19-amino acid signal peptide, an acidic 2-amino acid propeptide and a 226-amino acid catalytic domain. Tryptases comprise a family of trypsin-like serine proteases that are enzymatically active as heparin-stabilized tetramers. There are four functional genes for tryptase: å I, ∫ I, ∫ II and © I, which map to human chromosome 16p13.3, with ∫ tryptases representing the main isoenzymes expressed in mast cells. Mast cell proteases are a family of rodent protein homologs to human tryptases that are specifically expressed in mast cells and may serve as highly specific markers in the analysis of mast cell heterogeneity, differentiation and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Mast Cell Tryptase α, β and δ isoforms of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-275 representing full length Mast Cell Tryptase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-275 representing full length Mast Cell Tryptase of human origin |
| Entrez Gene | 7177 |
|---|
Application
Western blot analysis of TPSB2 expression in non-transfected 293T: (A), human TPSB2 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Mast Cell Tryptase expression in non-transfected: (A) and human Mast Cell Tryptase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of purified tryptase from human lung.| Application Notes | recommended for detection of Mast Cell Tryptase α, β and δ isoforms of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
