Application
Western blot analysis of TPX2 expression in K-562 (A) and Jurkat (B) whole cell lysates.| Product Name | TPX2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 448-747 mapping at the C-terminus of TPX2 of human origin |
| Modification Notes | TPX2 (targeting protein for Xklp2) is a microtubule-associated protein involved in targeting the motor protein Xklp2 to microtubules. Ran-GTP activates TPX2 for the chromatin-induced microbutule assembly during M phase. Aurora-A kinase associates with TPX2 at the spindle apparatus and may regulate TPX2 via phosphorylation during the spindle assembly. TPX2 appears to play a structural role in spindle formation. TPX2 activates Eg2 in a microtubule-dependent manner by stimulating the phosphorylation and kinase activity of Eg2. TPX2 is inactivated by binding to importin a, a nuclear import factor. Finally, the suppression of TPX2 with RNA interference causes defects in microtubule organization during mitosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TPX2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 448-747 mapping at the C-terminus of TPX2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 448-747 mapping at the C-terminus of TPX2 of human origin |
| Entrez Gene | 22974 |
|---|
Application
Western blot analysis of TPX2 expression in K-562 (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of TPX2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
