Application
Western blot analysis of NOD2 expression in RAW 264.7 whole cell lysate.| Product Name | NOD2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 611-910 mapping near the C-terminus of NOD2 of human origin |
| Modification Notes | The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), NOD1 (CARD4), and NOD2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg2+-binding site). NOD1 contains a putative regulatory domain and multiple leucine-rich repeats. NOD1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. NOD1 associates with the CARD-containing kinase RICK and activates NFkB. The self-association of NOD1 mediates proximity of RICK and the interaction of RICK with IKKg. In addition, NOD1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. NOD2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of NOD2 is highly restricted to monocytes, and activates NFkB in response to bacterial lipopoly-saccharides. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NOD2 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 611-910 mapping near the C-terminus of NOD2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 611-910 mapping near the C-terminus of NOD2 of human origin |
| Entrez Gene | 64127 |
|---|
Application
Western blot analysis of NOD2 expression in RAW 264.7 whole cell lysate.| Application Notes | recommended for detection of NOD2 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
