Application
Western blot analysis of PKA Iα/β reg expression in non-transfected 293T: (A), human PKA Iα/β reg transfected 293T: (B) and CCRF-CEM (C) whole cell lysates.Application
Western blot analysis of PKA Iα/β reg expression in SW-13 whole cell lysate.Application
Western blot analysis of PKA Iβ reg expression in non-transfected: (A) and mouse PKA Iβ reg transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PKA Iβ reg expression in non-transfected: (A) and human PKA Iβ reg transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PKA Iα reg expression in non-transfected: (A) and human PKA Iα reg transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PKA Iα reg expression in non-transfected: (A) and mouse PKA Iα reg transfected: (B) 293T whole cell lysates.| Product Name | PKA Iα/β reg Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 246-335 mapping within an internal region of PKA Iα reg of human origin |
| Modification Notes | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Four different PKA regulatory subunits have been identified, designated Iå, I∫, IIå and II∫. The PKA Iå reg protein is a tissue-specific extinguisher that downregulates the expression of seven liver genes in heptoma x fibroblast hybrids. Functional null mutations in the gene that codes for PKA Iå reg cause Carney complex (CNC). CNC is an autosomal dominant multiple neoplasia syndrome. CNC is associated with a variety of characterized symptoms such as cardiac and other myxomas, spotty skin pigmentation, endocrine tumors and psammamatous melanotic schwannomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PKA Iα and Iβ reg of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 246-335 mapping within an internal region of PKA I&alpha reg of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 246-335 mapping within an internal region of PKA Iα reg of human origin |
| Entrez Gene | 5573 |
|---|
Application
Western blot analysis of PKA Iα/β reg expression in non-transfected 293T: (A), human PKA Iα/β reg transfected 293T: (B) and CCRF-CEM (C) whole cell lysates.
Application
Western blot analysis of PKA Iα/β reg expression in SW-13 whole cell lysate.
Application
Western blot analysis of PKA Iβ reg expression in non-transfected: (A) and mouse PKA Iβ reg transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PKA Iβ reg expression in non-transfected: (A) and human PKA Iβ reg transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PKA Iα reg expression in non-transfected: (A) and human PKA Iα reg transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PKA Iα reg expression in non-transfected: (A) and mouse PKA Iα reg transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PKA Iα and Iβ reg of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
