Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of p115 expression in Hep G2 whole cell lysate.Application
Western blot analysis of p115 expression in rat liver tissue extract.| Product Name | p115 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of p115 of human origin |
| Modification Notes | The mammalian protein p115, known also as transcytosis-associated protein (TAP), tethering factor or vesicle docking protein (VDP), and its yeast homologue Uso1p have an essential role in membrane trafficking (1-3). p115 is phosphorylated in interphase but not in mitotic cells (2). Phosphorylated p115 is localized to the cytosol, whereas the unphosphorylated form is associated with membranes, mostly of the Golgi complex (1). Upon phosphorylation of p115 at Ser942, p115 is released from the membranes (2). In mammary glands, p115 synthesis is dependent of the stage of lactation (4). Both giantin and GM130 compete for binding to the C-terminal acidic domain of p115, and p115-giantin and p115-GM130 interactions mediate independent membrane tethering events (5). The amino terminal region of p115 is required for its localization to the Golgi (1). p115 is also expressed on transcytotic vesicles, where p115 is required for vesicle fusion with the target membrane and vesicular tubular clusters (6), which are involved in ER to Golgi transport (1). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a select set of SNAREs (7). p115 is a general factor acting within the secretory and endocytic pathways to bind transport vesicles prior to membrane fusion (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of p115 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of p115 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of p115 of human origin |
| Entrez Gene | 8615 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of p115 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of p115 expression in rat liver tissue extract.| Application Notes | recommended for detection of p115 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
