Application
Western blot analysis of HSP 40 expression in non-transfected: (A) and mouse HSP 40 transfected: (B) 293T whole cell lysates.Application
Western blot analysis ofHSP 40 expression in non-transfected: (A) and human HSP 40 transfected: (B) 293T whole cell lysates.| Product Name | HSP 40 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 241-340 mapping at the C-terminus of HSP 40 protein 1 of human origin |
| Modification Notes | Heat shock protein 40 (HSP 40) family proteins bind to HSP 70 through their J-domain and regulate the function of HSP 70 by stimulating HSP 70 ATPase activity. HSP 40, also known as DnaJ, functions together with DnaK (HSP 70) and GrpE as a molecular chaperone, involving them in assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. HSP 40 stimulates the association between HSC 70 and HIP and translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. There are five known HSP 40 family proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HSP 40 subfamily B member 1, and, to a lesser extent, HSP 40 subfamily B members 4 and 5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 241-340 mapping at the C-terminus of HSP 40 protein 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 241-340 mapping at the C-terminus of HSP 40 protein 1 of human origin |
| Entrez Gene | 3337 |
|---|
Application
Western blot analysis of HSP 40 expression in non-transfected: (A) and mouse HSP 40 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis ofHSP 40 expression in non-transfected: (A) and human HSP 40 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HSP 40 subfamily B member 1, and, to a lesser extent, HSP 40 subfamily B members 4 and 5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
