Application
Western blot analysis of Whirlin expression in non-transfected: (A) and human Whirlin transfected: (B) 293T whole cell lysates.| Product Name | Whirlin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 608-907 mapping at the C-terminus of Whirlin of human origin |
| Modification Notes | Whirlin is a cytoplasmic PDZ domain-containing protein that plays a role in elongation and maintenance of stereocilia, mechanosensory organelles located in hair cells of the inner ear. Whirlin co-localizes with actin filaments and is primarily detected in cochlear hair cells. It is connected to the dynamic Usher protein interactome and has a pleiotropic function in both the retina and the inner ear. Myosin XVa is a motor protein that accociates with the second and third PDZ domain of Whirlin through its C-terminal PDZ-ligand. Myosin XVa then delivers Whirlin to the tips of stereocilia, which are subsequently elongated. p55 also interacts with Whirlin, and mutations in DFNB31, the Whirlin gene, lead to an early ablation of p55 labeling of stereocilia, which may cause recessive hearing loss in rats and humans. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Whirlin, isoforms 1, 3 and 4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 608-907 mapping at the C-terminus of Whirlin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 608-907 mapping at the C-terminus of Whirlin of human origin |
Application
Western blot analysis of Whirlin expression in non-transfected: (A) and human Whirlin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Whirlin, isoforms 1, 3 and 4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
