Application
Western blot analysis of nucleoporin p62 expression in non-transfected 293T: (A), human nucleoporin p62 transfected 293T: (B), PC-3 (C) and HeLa (D) whole cell lysates.Application
Western blot analysis of nucleoporin p62 expression in non-transfected 293T: (A), mouse nucleoporin p62 transfected 293T: (B) and Daudi (C) whole cell lysates.Application
Western blot analysis of nucleoporin p62 expression in non-transfected 293T: (A), human nucleoporin p62 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of nucleoporin p62 expression in BJAB (A), HeLa (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of nucleoporin p62 in WR19L whole cell lysate (A) and human recombinant nucleoporin p62 fusion protein (B).| Product Name | nucleoporin p62 Antibody (H-122) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin å and karyopherin ∫ proteins. The karyopherin holoenzyme is a heterodimer of å and ∫ subunits. Karyopherin å functions in the recognition and targeting of substrates destined for nuclear import, while karyopherin ∫ serves as an adaptor, tethering the karyopherin å substrate complex to docking proteins (termed nucleoporins) on the nuclear envelope. p62 glycoprotein is one such nucleoporin, and is not only involved in the nuclear import of proteins, but also the export of nascent mRNA strands. An additional protein, NTF2 (nuclear transport factor 2), interacts with nucleoporin p62 as a homodimer and may be an obligate component of functional p62. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of nucleoporin p62 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 401-522 mapping at the C-terminus of nucleoporin p62 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 401-522 mapping at the C-terminus of nucleoporin p62 of human origin |
| Entrez Gene | 23636 |
|---|
Application
Western blot analysis of nucleoporin p62 expression in non-transfected 293T: (A), human nucleoporin p62 transfected 293T: (B), PC-3 (C) and HeLa (D) whole cell lysates.
Application
Western blot analysis of nucleoporin p62 expression in non-transfected 293T: (A), mouse nucleoporin p62 transfected 293T: (B) and Daudi (C) whole cell lysates.
Application
Western blot analysis of nucleoporin p62 expression in non-transfected 293T: (A), human nucleoporin p62 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of nucleoporin p62 expression in BJAB (A), HeLa (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of nucleoporin p62 in WR19L whole cell lysate (A) and human recombinant nucleoporin p62 fusion protein (B).| Application Notes | recommended for detection of nucleoporin p62 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
