Application
Western blot analysis of OPN expression in non-transfected: (A) and human OPN transfected: (B) 293T whole cell lysates.Application
Western blot analysis of OPN expression in non-transfected: (A) and human OPN transfected: (B) 293T whole cell lysates.Application
Western blot analysis of OPN expression in CCD-1064Sk whole cell lysate.| Product Name | OPN Antibody (FL-314) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of OPN of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-314 representing full length OPN of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-314 representing full length OPN of human origin |
| Entrez Gene | 6696 |
|---|
Application
Western blot analysis of OPN expression in non-transfected: (A) and human OPN transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of OPN expression in non-transfected: (A) and human OPN transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of OPN expression in CCD-1064Sk whole cell lysate.| Application Notes | recommended for detection of OPN of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
