Application
Western blot analysis of MD-2 expression in mouse spleen tissue extract.| Product Name | MD-2 Antibody (FL-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Lipopolysaccharide (LPS) is the principal proinflammatory component of the Gram-negative bacterial envelope (1). The lipopolysaccharide (LPS) receptor is a multi-protein complex that consists of at least three proteins, CD14, TLR4 and MD-2 (2). Each of these proteins are glycosylated. Specifically, MD-2 contains two N-linked glycosylation sites at positions Asn(26) and Asn(114) (2). MD-2 is indispensable for TLR4-dependent LPS responses because cells expressing TLR4/MD-2, but not TLR4 alone, respond to LPS (3). Intestinal epithelial cells (IEC) express low levels of TLR4 and MD-2 and are LPS unresponsive (4). T cell-derived cytokines lead to increased expression of TLR4 and MD-2, and LPS-dependent pro-inflammatory cytokine secretion in IEC (4). The human LY96 gene maps to chromosome 8q21.11 and encodes a 162 amino acid protein with a predicted 16-amino acid signal peptide (5,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MD-2 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-160 representing full length MD-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-160 representing full length MD-2 of human origin |
| Entrez Gene | 23643 |
|---|
Application
Western blot analysis of MD-2 expression in mouse spleen tissue extract.| Application Notes | recommended for detection of MD-2 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
