Application
Western blot analysis of RANK expression in non-transfected: (A) and mouse RANK transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing membrane and cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of RANK expression in Hep G2 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed SJRH30 cells showing membrane localization.| Product Name | RANK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Members of the tumor necrosis factor (TNF) receptor superfamily interact with signaling molecules of the TNF receptor-associated factor (TRAF) family to activate the NF˚B and JNK pathways. RANK (receptor activator of NF˚B) is a member of the TNFR family identified on dendritic cells. This type I membrane receptor is expressed in a broad range of tissues. The C-terminus of RANK is required for RANK to bind TRAF2, 5 and 6, and it is also necessary for stimulating NF˚B activation. The ligand for this receptor, RANKL (also designated TRANCE or ODF), is a type II transmembrane protein expressed primarily in lymphoid tissues and T cell lines. RANKL appears to be an important regulator of T cells and osteoclasts. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RANK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 317-616 of RANK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 317-616 of RANK of human origin |
| Entrez Gene | 8792 |
|---|
Application
Western blot analysis of RANK expression in non-transfected: (A) and mouse RANK transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing membrane and cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of RANK expression in Hep G2 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed SJRH30 cells showing membrane localization.| Application Notes | recommended for detection of RANK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
