Application
Western blot analysis of Wnt-15 expression in non-transfected: (A) and human Wnt-15 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | Wnt-15 Antibody (H-94) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Wnt genes encode a family of secreted extracellular signaling glycoproteins, which function in a variety of important developmental processes such as regulation of cell growth and differentiation. Wnt proteins also play roles in carcinogenesis. Wnt-14 rather than Wnt-15 is preferentially expressed in various types of human cancer and is upregulated by IFN-g, but not by TNFa, in cells derived from gastric cancer. Wnt-15 is expressed in fetal and adult kidney and is most homologous to Wnt-14. Wnt-16, another member in the Wnt family, has two mRNA isoforms, Wnt-16a and Wnt-16b. These isoforms differ in the composition of their 5'UTR and first exon, which results in differential expression. Wnt-16a is expressed only on the pancreas, whereas Wnt-16b is highly expressed in adult kidney, placenta, brain, heart and spleen, but not in bone marrow. However, Wnt-16 transcripts are present in bone marrow and cell lines derived from pre-B acute lymphoblastoid leukemias patients carrying the E2A-Pbx1 hybrid gene. Thus, WNT-16 is a downstream target of E2A-Pbx1, and the Wnt-16-mediated autocrine growth mechanism may contribute to the development of t(1;19) pre-B acute lymphoblastoid leukemias. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Wnt-15 and Wnt-14B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 216-335 mapping near the N-terminus of Wnt-15 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 216-335 mapping near the N-terminus of Wnt-15 of human origin |
| Entrez Gene | 7484 |
|---|
Application
Western blot analysis of Wnt-15 expression in non-transfected: (A) and human Wnt-15 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Wnt-15 and Wnt-14B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
